Isolation of rheumatoid factors
نویسندگان
چکیده
منابع مشابه
Studies on the isolation of rheumatoid factor.
The need for the isolation of rheumatoid factor in a high state of purity and in sufficient quantity for antiserum production has led to an appraisal of the various purification methods available. Ziff, Brown, Lospalluto, Badin, and McEwen (1956) demonstrated that the sensitized sheep cell agglutinating activity of rheumatoid serum was precipitated in the euglobulin fraction, and Svartz and Sch...
متن کاملIsolation and characterization of a light chain variable region gene for human rheumatoid factors
Previously, we isolated a Vk gene (Humkv325) from a human placenta that encodes RF light chains bearing the PSL2 and PSL3 CRI markers. Here we report the isolation and characterization of a second human Vk gene (Humkv328) that can be used for RF synthesis. This Vk gene probably encodes at least two 6B6.6 CRI+ RF light chains (Les and Pom) from unrelated subjects, and thus may be related to the ...
متن کاملRheumatoid factors specific for active rheumatoid arthritis.
To measure rheumatoid factors specific for patients with rheumatoid arthritis an enzyme linked immunosorbent assay (ELISA) was developed to measure rheumatoid factors in human serum that bind a cross-reactive determinant shared on human and other mammalian IgG. Rheumatoid factors that cross link human IgG and sheep IgG in a double binding ELISA were almost completely specific (greater than 99%)...
متن کاملRheumatoid factors in patients with rheumatoid arthritis.
180 Canadian Family Physician • Le Médecin de famille canadien d VOL 52: FEBRUARY • FÉVRIER 2006 I is important for family physicians to understand the role of rheumatoid factor (RF) in management of patients with rheumatoid arthritis (RA). Rheumatoid factors are antibodies that recognize the Fc portion of IgG molecules as their antigens; RFs can be of any immunoglobulin isotype (IgM, IgG, IgE)...
متن کاملRheumatoid factors in families.
Ziff, Schmid, Lewis, and Tanner (1958) first reported that rheumatoid factors reacting with human Cohn fraction II gamma globulins assessed by means of a latex-fixation test (LEI) were aggregated in the families of hospital patients with rheumatoid arthritis. This evidence for familial aggregation was confirmed by Ball and Lawrence (1961) in the urban population sample of Leigh, England, the te...
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ژورنال
عنوان ژورنال: Arthritis & Rheumatism
سال: 1961
ISSN: 0004-3591,1529-0131
DOI: 10.1002/art.1780040204